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您的位置:代理品牌>> Epicentre
Epicentre Biotechnologies是美國分子生物學產品專業生產商,其在基因組研究、建庫,蛋白研究和RNA分析方面都有自己的特色產品。CopyControl文庫系列產品聞名于世。轉座子系統也是其特色產品。另外還有SNP突變檢測、測序、DNA指紋圖譜分析方面的產品,涉及面廣。
 
 

基因文庫構建相關產品Genomic Library Production

Genomic Library Production

A Brief Guide to Genomic Library Production 基因文庫產品選擇指南

Fosmid Cloning Kits

·         CopyControl™ Fosmid Library Production Kit
CopyControl™ HTP Fosmid Library Production Kit

·         EpiFOS™ Fosmid Library Production Kit

·         CopyControl™ BAC Autoinduction Solution
CopyControl™ Fosmid Autoinduction Solution

Fosmid Clone Purification

·         FosmidMAX™ DNA Purification Kit

Bacterial Artificial Chromosome (BAC) Cloning Kits

·         CopyControl™ BAC Cloning Kits (BamH I, EcoR I, or Hind III)
CopyControl™ pCC1BAC™ Cloning-Ready Vectors (BamH I, EcoR I, or Hind III)

·         pIndigoBAC-5 (BamH I Cloning-Ready) Vector 
pIndigoBAC-5 (Hind III Cloning-Ready) Vector

Converting Single-Copy BAC or Fosmid Clones to Multi-Copy-Inducible 轉換單拷貝至誘導多拷貝中

·         EZ-Tn5™ <oriV/KAN-2> Insertion Kit 轉座插入試劑盒

BAC Clone Purification

·         BACMAX™ DNA Purification Kit

·         BACMAX96™ DNA Purification Kit

Sizing BAC Clones 篩查BAC克隆

·         BAC-Tracker™ Supercoiled DNA Ladder   超螺旋DNA分子量標準

Cosmid Cloning Kits

·         pWEB-TNC™ Cosmid Cloning Kit 
pWEB™ Cosmid Cloning Kit

Phage T1-Resistant Competent Cells 感受態細胞

·         Phage T1-resistant TransforMax™ EPI300™-T1R Electrocompetent E. coli and TransforMax™ EPI300™-T1R Chemically Competent E. coli

·         Phage T1-resistant TransforMax™ EC100™-T1R Electrocompetent E. coli

High-Efficiency Lambda Packaging Extracts 高效Lambda包裝試劑盒

·         MaxPlax™ Lambda Packaging Extracts

 

Epicentre產品Competent Cells 試劑盒

TransforMax EPI300 Electrocompetent E.coli

Applications

·         Generation of inducible copy-number clones using the CopyControl™ Cloning System.

 

TransforMax™ EPI300™ E. coli cells lack the tonA gene and are engineered for use with EPICENTRE's CopyControl™ cDNA, Gene, and PCR Cloning Kit and other CopyControl Cloning Systems* that do not require phage T1-resistant cells. The cells contain an inducible mutant trfA gene whose gene product is required for initiation of replication from the oriV origin of replication, such as that in CopyControl pCC1™ vectors or in clones that are retrofitted with CopyControl capability using theEZ-Tn5™<oriV/KAN-2> Insertion Kit. On LB chloramphenicol plates or in LB or SOC media supplemented with chloramphenicol, CopyControl clones grown in TransforMax EPI300 E. coli replicate at single-copy number from the F-factor replicon because expression of the trfA gene is repressed. Addition of CopyControl Induction Solution induces the cells to express the trfA gene product and induces their replication at high-copy number from oriV (Fig. 1). Replication from oriV results in higher yields and higher purity of cloned DNA.

Benefits

·         Copy-number of clones is under tight control of an inducible promoter linked to thetrfA gene.

·         High transformation efficiency with clones of all sizes.

·         lacZΔM15 for blue/white screening of recombinants.

·         Restriction-minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] phenotype enables efficient cloning of methylated DNA.

·         Endonuclease-minus (endA1), to ensure high yields of DNA.

·         Recombination-minus (recA1), for greater stability of large insert clones.

 Figure 1. Copy number of CopyControl™ BAC clones can be induced 10- to 20-fold in TransforMax™ EPI300™ E. coli. The yield of BAC DNA from CopyControl BAC clones of 110-145 kb increased >14-fold following addition of CopyControl Induction Solution. U=uninduced cells; I=induced cells.

 

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ-rpsL (StrR) nupG trfA dhfr

TransforMax EPI300 Electrocompetent E. coli

·         Transformation efficiency of >1 x 1010 cfu/µg of pUC19.

TransforMax EPI300 Chemically Competent E. coli

·         Transformation efficiency of >5 x 108 cfu/µg of pUC19.

*Covered by issued and/or pending patents.

 

Catalog No.

Concentration

Size


TransforMax™ EPI300™ Electrocompetent E. coli

EC300105

 

5 X 100 µl,

EC300110

 

10 X 100 µl

EC300150

 

50 X 100 µl

Includes CopyControl™ Induction Solution and pUC19 control DNA.


TransforMax™ EPI300™ Chemically Competent E. coli

C300C105

 

10 X 50 µl

Includes CopyControl™ Induction Solution and pUC19 control DNA.


CopyControl™ Induction Solution

CCIS125

 

25 ml

1,000X concentrated solution. Filter sterilized.

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